Science and Engineering 601
Fayetteville, AR 72701
Ph.D. University Miami, Rosenstiel School of Marine and Atmospheric Science, 1993
Research projects in my lab are divided into two intertwined themes; 1) Protist phylogeny and 2) the origin, evolution, compartmentalization of eukaryotic anaerobic metabolism. Single cell eukaryotes comprise the majority of eukaryotic diversity yet the relationships among protist lineages is still controversial. We are committed to constructing robust eukaryote phylogenies using a variety of genes (singly or in combination). Various aerobic and anaerobic amoebae and flagellates are being studied as well as some very obscure protist parasites. It is becoming increasingly evident that a key event in the evolution of eukaryotes was the origin of mitochondria from endosymbiotic-proteobacterial precursors. Molecular phylogenies and subcellular localization studies are providing stronger and stronger evidence that all extant eukaryotes have experienced the ancestral mitochondrial endosymbiosis, even ‘amitochondriate’ anaerobes. We are searching for direct genetic evidence (mitochondrial marker genes) and genes for core enzymes of mitochondrial relic or related organelles found in these anaerobes. We hope to clarify the evolutionary links between the mitochondria and their anaerobic counterparts.
Molecular phylogeny of eukaryotes to determine relationships within and among major protistan lines of descent, and assessing the contribution of prokaryotic genetic material that led to the creation of the eukaryotic cell and its integral components.
Dr. Silberman's lab website is under construction.
Yubuki N., Leander B. S., Silberman J. D. 2010. Ultrastructure and molecular phylogenetic position of a novel phagotrophic stramenopile from low oxygen environments: Rictus lutensis gen. et sp. nov. (Bicosoecida, incertae sedis). Protist, in press.
Brown M. W., Spiegel F. W., Silberman J. D. 2009. Phylogeny of the “forgotten” cellular slime mold, Fonticula alba, reveals a key evolutionary branch within Opisthokonta. Mol. Biol. Evol. 26(1):2699-2709.
Shadwick L. L., Spiegel F. W., Shadwick J. D., Brown M. W., Silberman J. D. 2009. Eumycetozoa = Amoebozoa?: SSU rDNA phylogeny of protosteloid slime molds and its significance for the Amoebozoan supergroup. PLoS ONE 4(8):e6754.
Minge M. A.,
Silberman J. D., Orr R. J., Cavalier-Smith T., Shalchian-Tabrizi K., Burki F., Skjælend
A., Jakobsen K. S. 2009. Evolutionary position of breviate amoebae and the primary
eukaryote divergence. Proc. R. Soc. B. 276(1657): 597-604.
Minge M. A., Silberman J. D., Orr R. J., Cavalier-Smith T., Shalchian-Tabrizi K., Burki F., Skjælend A., Jakobsen K. S. 2009. Evolutionary position of breviate amoebae and the primary eukaryote divergence. Proc. R. Soc. B. 276(1657): 597-604.
Hampl V., Silberman J. D., Stechmann A., Diaz-Trivino S., Johnson P. J,. Roger A. J. 2008. Genetic evidence for a mitochondriate ancestry in the "amitochondriate" flagellate Trimastix pyriformis. PLoS ONE 3(1):e1383.