Home » Faculty & Staff » Du, Yuchun

Du, Yuchun

Du, Yuchun

Office: SCEN 528
Phone: 575-6944
ydu@uark.edu

Degrees:

Ph.D. Kagoshima University, 1998

Research Interests:

Mass spectrometry based quantitative proteomics involves using stable isotope to differentially label proteins or peptides, and mass spectrometry to compare the relative abundance of the proteins in different samples. Various novel quantitative proteomics technologies have been developed to compare protein expression changes, analyze protein-protein/protein-drug interactions, and determine the post-translational modification states. Research in my laboratory is concentrated in the following two areas:

1.  Subcellular proteomics: We are using quantitative proteomic approaches (AACT/SILAC, and ICAT) to systematically compare the protein expression changes at the subcellular level (such as mitochondria) between normal and disease tissues/cells. The objective is to identify the proteins that alter cellular process.

2.  Protein complexes: We are using multidisciplinary approaches including techniques from quantitative proteomics, biochemistry and cell biology to identify and characterize protein complexes that play critical roles in cell proliferation or programmed cell death.


Academic Interests:

Mass spectrometry, quantitative proteomics, subcellular proteomics, mammalian cell biology, protein-protein interactions

Lab Website:

under construction

Recent Publications:

Du Y.-C., Gu S., Zhou J., Wang T., Cai H., MacInnes M. A., Bradbury E. M., and Chen X. 2006. The dynamic alterations of H2AX complex during DNA repair detected by a proteomic approach reveal the critical roles of Ca2+/calmodulin in the ionizing radiation induced cell cycle arrest. Molecular & Cellular Proteomics 5: 1033-1044.

Wang T., Chuang T., Ronni T., Gu S., Du Y.-C., Sun H., Yin H. L., Cai H., and Chen X. 2006. Fliih negatively modulates the MyD88-dependent pathway. J. Immunology 176: 1355-1362.

Liu Z, Lu H., Shi H., Du Y-C., Yu J., Gu S., Chen X., Liu K.J., and Hu C.A. 2005. PUMA overexpression induces reactive oxygen species generation and proteosome-mediated stathmin degradation in colorectal cancer cells. Cancer Res. 65: 1647-1654.

Gu, S, Du Y.-C., Chen J., Liu Z., Hu C., and Chen X. 2004. Large-scale quantitative proteomic study of PUMA-induced apoptosis using two-dimensional liquid chromatography–mass spectrometry coupled with amino acid coded mass tagging. J. Proteome Res. 3: 1191-1200.

Du Y.-C., Hong S., and Spreitzer R. J. 2000.  RbcS  suppressor mutations improve the thermal stability and CO2/O2 specificity of rbcL-mutant ribulose-1,5-bisphosphatecarboxylase/oxygenase. Proc. Natl. Acad. Sci. USA 97: 14206-14211.