Arkansas Cooperative Fish and Wildlife Research Unit
AR Coop Address: SCEN 622, University of Arkansas
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Active/Completed Projects

 

WILDLIFE MANAGEMENT

 

Distribution of the king rail (Rallus elegans) along the Mississippi Flyway

Funding Source: U.S. Fish and Wildlife Service
Project Duration: Spring 2006 - Fall 2007
Principle Investigator: DAVID G. KREMENTZ
Graduate Research Assistant: ABIGAIL J. DARRAH (M.S. student)

Research Objectives:

1. Determine the distribution and abundance of king rails along the Mississippi River in Missouri, between St. Louis and Hannibal
2. Determine habitat use of king rails, including nesting and brood-rearing habitats

Management Implications:

1. Establish guidelines for managing wetland habitat for king rails
2. Modify hunting regulations if necessary to avoid harvest of endangered migratory populations of king rails on their wintering grounds


Project Summary:

King rail populations have declined dramatically over the last 30 years, largely due to loss of wetland habitat. Declines have been greatest in the migratory populations of the midwest, and the species is considered threatened or endangered in some states. However, the king rail is a game bird in many Atlantic and Gulf coast states, where the resident populations are thought to be stable. The migration routes and wintering grounds of the migratory king rails are not well known, thus it is possible that these endangered populations are exposed to harvest in the winter.

We began a study on king rails along the Mississippi River in Missouri in May 2006. Because of the secretive nature and low population density of the king rail, we surveyed each site 8-10 times during the 2006 and 2007 seasons. We had very few responses in both years, mostly early in the season on 2-3 out of 15 wetlands. At each survey point we collected cover type information within 50 m and percent forest within 400 m. We searched for nests and broods throughout each season, and located 3 inactive nests and 6 broods in 2006, and 5 broods in 2007. In 2007 we also banded and placed transmitters on several adult and juvenile king rails. For all nests, brood locations, and radio point locations, we took habitat information including water depth, vegetation cover, and dominant plant species within a 5 m radius of each point.

We will analyze the data using the program PRESENCE, which independently estimates detection probability (p) and probability of site occupancy (?). Using the survey data and the habitat covariates, we will select the best model predicting king rail occupancy of wetland sites. For nest, brood, and telemetry habitat data, we will use resource selection functions to determine how king rails use the available wetland habitats in our study area.

 

 

Survey of Breeding Secretive Marsh Birds in the Delta Region of Arkansas

Funding Source: Arkansas Game and Fish Commission
Project Duration: Spring 2005 - spring 2007
Principal Investigator: DR. DAVID G. KREMENTZ
Graduate Research Assistant: MICHAEL J. BUDD (M.S. student)

Research Objectives are to Determine:

1) Which secretive marsh birds occur in the Delta of Arkansas during the breeding season
2) What proportion of sites in the Delta are occupied by secretive marsh birds
3) What is the probability of detecting a secretive marsh bird in the Delta
4) What factors affect detecting secretive marsh birds
5) Basis habitat types occupied by secretive marsh birds

Management Implications:

1) Establish baseline data on basic habitat types
2) Habitat management recommendations to benefit marsh birds
3) Establish information on sites used primarily by marsh birds


Project Summary:

The Delta Region of Arkansas was once part of a vast wetland area comprised of mostly bottomland hardwoods as well as emergent, and submergent wetlands, and prairie. The Mississippi Alluvial Valley of Arkansas is part of the alluvial floodplain of the lower Mississippi River that extends through Arkansas, Louisiana, and Mississippi with smaller segments located in Tennessee, Missouri, Illinois, and Kentucky. Before European settlement, the LMAV was a 10 million-ha, forested-wetland system. Between the 1950s and the 1970s, much of this land was cleared and converted to agriculture and aquaculture facilities. Along with this change in land use has been an unknown change in the use of those wetlands by secretive marsh birds. Secretive marsh birds include all species that primarily inhabit marshes (i.e., marsh-dependent species). Primary species of concern in North America include the King Rail, Clapper Rail, Virginia Rail, Sora, Black Rail, Yellow Rail, American Bittern, Least Bittern, Pied-billed Grebe, Purple Gallinule, and Common Moorhen.

During the summer of 2005 we surveyed 80 wetlands and approximately half of these were occupied by = 1 secretive marsh bird. King rails occupied 12 sites with more than one bird being observed at each site. Virginia rails were one of the least common marsh birds, being detected at only 5 sites. Soras were very common early in the summer and were detected at 19 sites. We encountered 9 American bitterns early in the season. Least bitterns occupied 14 sites, Pied-billed grebes occupied 9 sites, and purple gallinules and common moorhens only occupied 2 sites.

 

 

American Woodcock Fall Migration Ecology in the Central Region

Funding Source: U.S. Fish and Wildlife Service
Project Duration: Fall 2001 – Fall 2004
Principle Investigator: DR. DAVID KREMENTZ
Graduate Research Assistant: NICK MYATT (M.S. student)

Research Objectives:

1) Document American woodcock fall migration routes in the Central Region
2) Establish the timing of woodcock fall migration
3) Investigate woodcock habitat use during fall migration

Management Implications:

1) Establish baseline data on American woodcock fall migration ecology
2) Habitat management recommendations to benefit migrating American woodcock
3) Establish information on primary woodcock wintering areas and habitats<


Project Summary:

Despite decreased hunter bag limits and shortening of the hunting season, American woodcock populations have been declining since singing ground surveys began in 1968. Woodcock have been studied extensively on the breeding grounds and to a lesser extent on the wintering grounds, but virtually no research has been conducted on the specie’s fall migration ecology. We know roughly when woodcock leave the breeding grounds and when they arrive on the wintering grounds, but little is known about the period in between.

In the fall of 2001 we began a 3 year study to document American woodcock fall migration routes, timing, and habitat use in the Central Region. In fall 2002, 360 woodcock were captured and fitted them with radio transmitters in Minnesota, Wisconsin, and Michigan, of which 274 successfully began fall migration. Radio-marked woodcock were relocated during migration and on the wintering grounds. During fall migration we conduct aerial searches for radio-marked woodcock in Illinois, Missouri, Kansas, Oklahoma, Arkansas, Kentucky, Tennessee, Mississippi, Louisiana, and Texas. During 125 hours of aerial telemetry, we found 29 possible radio-marked woodcock locations. We confirmed 22 of these locations by flushing the radio-marked bird while the remaining 7 locations were unconfirmed due to lack of access or the absence of the radioed bird when we arrived. Of the 22 confirmed birds, 2 were located in bottomland forest, 13 in pine forests, and 7 in upland hardwood forests. We performed 838 50-m ground transect surveys in 26 different forest types to document habitat use by woodcock. We flushed 38 woodcock over a total of 41.9 km of transects using a flushing dog.

 

 

Impacts of Harvest Management on the Age and Sex Structure of White-Tailed Deer (Odocoileus virginianus) Populations in Arkansas


Funding Source: Arkansas Game and Fish Commission
Project Duration: 1 July 2000 to 31 September 2004
Principal Investigator: DAVID G. KREMENTZ
Graduate Research Assistant: BRET A. COLLIER (Ph.D. Student)

Research Objectives:

1. Determine impact of harvest management strategies (e.g. antler point restrictions) on sex and age structure of white-tailed deer harvest in Arkansas.
2. Determine if harvest management strategies influence white-tailed deer harvests differently dependent upon spatial scale.
3. Investigate the human dimension of statewide white-tailed deer management practices.

Management Implications:

1. Establish management recommendations based on the impact of harvest management strategies in Arkansas.
2. Verify the effectiveness of harvest management strategies and determine if harvest differences between states under those strategies and those states that are not.
3. Gather baseline information on how AGFC can assist private landowners managing white-tailed deer.
4. Estimate at which spatial scale harvest management strategies influence deer demography.


Project Summary:

We are investigating the impact of a statewide antler restriction on the age structure and sex ratio of white-tailed deer (Odocoileus virginianus) harvested in Arkansas between 1984-2003. Our objectives are 1) to determine which factors have the most influence on white-tailed deer population demography pre- and post the establishment of a statewide antler restriction, 2) determine whether influence of the antler restriction is scale dependent and 3) to gather demographic information on white-tailed deer hunters in Arkansas. During fall 2000 and fall 2001, we surveyed registered white-tailed deer hunting camps in Arkansas to gather information on harvest and habitat management practices currently in use across Arkansas. During summer 2001 and summer 2002, we surveyed Arkansas hunting license purchasers to gather information regarding white-tailed deer hunter demographics, harvest preferences, opinions, and concerns. Using decision analysis theory, we are modeling the impact of the statewide antler restriction on white-tailed deer at different spatial scales in Arkansas. Decision analysis allows us to link available harvest data with key biological processes underlying the population dynamics of white-tailed deer in Arkansas. We will assess relations among many factors influencing white-tailed deer harvest structure and underlying population dynamics.

 

 

The Importance of Lower Mississippi River Alluvial Valley Reforestation and Wetland Restoration Sites to Wintering Migratory Birds

Funding Sources: U.S. Fish and Wildlife Service, USGS - BRD
Project Duration: September 1999 to June 2004
Principal Investigator: DAVID G. KREMENTZ
Graduate Research Assistant: ROBERT H. DOSTER (Ph.D. Student)

Research Objectives:

1. Determine the species composition and population density of migrant birds wintering in and utilizing early- successional vegetation sites in the Lower Mississippi River Alluvial Valley.
2. Characterize the vegetation in early-successional fields and relate these vegetative characters to bird species richness and abundance.
3. Focus on three uncommon wintering grassland bird species (Short-eared Owl, Sedge Wren and Le Conte's Sparrow) by determining more specific wintering habitat requirements for purposes of conservation management.
4. Determine the breeding origin of selected bird species wintering in the Lower Mississippi River Alluvial Valley, through analysis of stable isotopes incorporated in feathers, for use in conservation planning between breeding and wintering sites.

Management Implications:

1. Compare treatments (management practices) between study sites and determine which management regime is most beneficial to the greatest number of species and to those species that are in particular need of conservation attention.
2. Formulate management recommendations based on research objectives for the purpose of a region-wide conservation strategy aimed at providing optimal wintering habitat for the greatest number of short-distance migrant bird species.


Project Summary:

Significant efforts to restore bottomland hardwood forests and associated wetlands have been undertaken throughout the Lower Mississippi River Alluvial Valley (LMAV) in recent years. These restoration activities have resulted in large acreages of early-successional habitats throughout the region. Early-successional habitats are utilized by a number of grassland bird species as winter refugia. Considering that grassland birds, as a whole, are experiencing population declines and in view of the fact that little research has been done on their winter ecology, we are investigating the role that these restored habitats within the LMAV play for this group of birds. In total, 69 sites throughout the LMAV of Arkansas, Louisiana and Mississippi were surveyed in winter 2000, 2001 and 2002 for bird species richness, abundance and distribution. Associated measurements of vegetation structure were also collected for all sites. In addition, species-specific habitat measurements were made for three species of concern within the region: Short-eared Owl (Asio flammeus), Sedge Wren (Cistothorus platensis) and Le Conte's Sparrow (Ammodramus leconteii). Analysis of the resulting population data for all sites and bird species, the relationship of vegetation structure and landscape parameters, and the specific winter habitat preferences of species of concern are ongoing.

 

 

Turnover Rates of Pectoral Sandpipers during Fall Migration
in the Lower Mississippi Alluvial Valley

Funding Source: US Fish and Wildlife Service, Arkansas Game and Fish Commission
Project Duration: July 2001 to June 2003
Principle Investigator: DAVID G. KREMENTZ
Graduate Research Assistant: SARAH E. LEHNEN (M.S. Student)

Research Objectives:

1. Estimate turnover rates of pectoral sandpipers (Calidris melanotos) during fall migration in the Lower Mississippi Alluvial Valley.

Management Implications:

1. Turnover rate estimates will be used to estimate shorebird use days during the fall migration in the Lower Mississippi Alluvial Valley.
2. These estimates will be valuable in managing fall shorebird habitats in the Lower Mississippi Alluvial Valley.


Project Summary:

We attached radio transmitters to 39 pectoral sandpipers (Calidris melanotos) during the fall of 2001at 3 sites in Arkansas. Nineteen transmitters were attached to birds at Bald Knob National Wildlife Refuge (Bald Knob), 7 at the Small Farm Outreach and Water Management Center of the University of Arkansas-Pine Bluff (Small Farm), and 13 at Overflow National Wildlife Refuge (Overflow). Our objective was to determine the average turnover rate of shorebirds moving through the Lower Mississippi Alluvial Valley during fall migration using the pectoral sandpiper as an example of a typical migrant. The birds were tagged and tracked between August 1 and September 28 of 2001. The mean length of time monitored for birds with transmitters was 4.3 (3.23 SE), 1.2 (0.3 SE), and 4.2 (2.83 SE) days for Bald Knob, Small Farm, and Overflow, respectively. Small Farm, an aquacultural facility, was believed to have a shorter period of monitored stay due to the brief duration of available habitat at the site. This is a possible illustration of the differences between habitat managed for shorebirds and aquacultural facilities. We will attempt to further investigate this matter in the 2002 field season.

We plan to attach 75 transmitters to pectoral sandpipers during the fall of 2002. In addition to the same 3 sites used in 2001, we will add Yazoo National Wildlife Refuge in Mississippi. We will also use aerial tracking to locate the birds after they leave the banding areas and set up an automated tracking unit at Catahoula Lake in Louisiana to record any birds with transmitters using that stopover site. During the fall of 2002, we will also color mark least sandpipers (Calidris minutilla) and use mark resight data to estimate the turnover rate for this species.

 

 

Short-term Responses of Grassland Bird Populations to Native Grass Conversion and Mowing in Northwest Arkansas

Funding Source: AR Game and Fish Commission; USGS-BRD, CFWRU
Project Duration: August 2001 to May 2004
Principal Investigator: WILLIAM L. THOMPSON
Graduate Research Assistant: JASON D. LUSCIER (M.S. Student)

Research Objectives:

1. Evaluate short-term effects of native grass conversion activities (early-spring burning, mowing, disking, and herbicide application) and spring/summer mowing on species richness and nest success of grassland bird populations in the Wedington Unit of Ozark National Forest in Northwest Arkansas

Management Implications:

1. Provides insights into short-term effects of grassland restoration and spring/summer mowing on grassland bird species and communities, which may be implemented in larger-scale conservation strategies (e.g., Partners in Flight) and may lead to improved management of these species.


Project Summary:

Many grassland bird species have suffered serious declines over the past several decades, primarily due to habitat loss and degradation. A better understanding of effects of land management practices on grassland birds may lead to improved management of these species. Therefore, we are evaluating short-term effects of native grass conversion activities (early-spring burning, mowing, disking, and herbicide application) and spring/summer mowing on species richness and nest success of grassland bird populations in the Wedington Unit of Ozark National Forest in Northwest Arkansas during 2002-2003. Native grass conversion activities were suspended due to litigation during Spring 2002, although burning was conducted on 1 pasture prior to suspension. Consequently, we estimated species richness and nest success in 5 pastures during April - July 2002: a reference pasture with no treatment, an early-mowed pasture (mowed 5/19/02), a mid-mowed pasture (mowed 6/01/02), a late-mowed pasture (mowed 7/10/02), and an early-burned pasture (burned 4/09/02). Preliminary analyses indicated that maximum species richness after 15 May (i.e., resident breeders only) was lowest in the early-burned pasture (22 [SE = 4]) and highest in the early-mowed pasture (40 [SE = 10). Maximum species richness was intermediate in the mid-mowed (28 [SE = 4]), late-mowed (29 [SE = 5]), and reference (30 [SE = 5]) pastures. Naive estimates of nest success ranged from 6% (1/17) in the mid-mowed pasture to 67% (6/9) in the reference pasture. Field sparrow (Spizella pusilla) was the most commonly detected nesting species (47% of 54 nests). Next year's research will include reference, native-grass conversion (if available), and mowed pastures, with cattle-grazed pastures added if feasible.

 

 

FISHERIES

 

Spatial and temporal variation in the effects of grazing fish and crayfish on benthic communities during stream drying

Funding Source: Causey-Grant in Aid
Project Duration: August 2004 to December 2009
Principal Investigator: DANIEL D. MAGOULICK
Graduate Research Assistant: JOHN P. LUDLAM (Ph.D. Student)

Objectives:

1. Quantify effects of stream crayfish and central stoneroller minnows on stream structure and function in the Little Mulberry River, AR.
2. Determine how stream drying influences the ability of grazers to affect benthic communities.

1. Information gained from this research will show how crayfish and central stonerollers affect the functioning of headwater streams in the Boston Mountain ecoregion.
2. Results will help develop predictions regarding the importance of stream drying to within-stream processes.


Project Summary:

Central stonerollers (Campostoma anomalum) and crayfish (Orconectes meeki) are common in the Boston Mountains of Arkansas and can have substantial effects on the abundance and composition of algae, deposited sediment, and invertebrate communities, thereby altering important aspects of stream ecosystem functioning. Summer stream drying is extensive in these systems and may alter interactions between grazers and benthic communities. I am using a combination of experiments in laboratory mesocosms and a natural stream to study how environmental variation alters the role of grazers in stream functioning. Exclusion quadrats in the Little Mulberry River were used to manipulate grazer densities during 2006 and 2007 to quantify the spatial and temporal variation in grazer effects among pools and across seasons and years, and relate this variation to environmental conditions. Results suggest that the frequency of flood events and grazer density are critical to the magnitude of grazer effects. Laboratory mesocosms are now being used to test these hypotheses. Understanding the relationship between stream drying and the interaction of stream functioning and grazers is critical to predicting how future increases in hydrological variation due to climate change will impact stream processes.

 

Effects of Otter (Lontra canadensis) Predation on Stream Communities

Principal Investigator: DANIEL D. MAGOULICK
Graduate Research Assistant: MATTHEW P. DEKAR (Ph.D. Candidate)

Objectives:

1. Quantify aquatic and riparian food webs and investigate temporal and spatial variation in predator-prey dynamics between otters and the aquatic community.
2. Quantify otter diet and develop a bioenergetics model to estimate the amount of each prey type consumed.
3.Use experimental manipulations to test hypotheses regarding the impact of otter predation on stream communities.

Management Implications:

1. Results will provide information regarding the impact of otters on stream communities, including potential impact to sport fishes.
2. Bioenergetics modeling will give insight into ecological constraints regulating otter populations.

3. This study will enable predictions concerning how predator and prey populations will respond to environmental variation associated with seasonal fluctuations in water levels, and habitat degradation and fragmentation.


Project Summary:

In the Ozark Mountains of northwest Arkansas, river otters (Lontra canadensis) may be an important and overlooked predator in stream communities. In particular, there is growing concern in this region that otters both prey upon and compete with important sport fishes. Therefore, our objectives are to examine the effects of river otter predation on the abundance and distribution of fish and crayfish in Little Mulberry Creek and the Mulberry River. From 2005 through 2007, we sampled possible otter prey items for stable isotope analysis and food web development. In addition, we sampled otter fecal matter in the field and we obtained stomachs from otter carcasses donated during the winter trapping season. In 2006, we began blood sampling from live-captured individuals to supplement scat and stomach contents data. In addition, we conducted crayfish tethering experiments to assess crayfish mortality associated with terrestrial and aquatic predators. Results indicated that otters rely heavily on crayfish prey during the summer months and switch to fish during the winter. We are continuing the diet analyses and are beginning to develop a bioenergetics model to estimate the amount of each prey type consumed. In addition, we are beginning crayfish population monitoring and laboratory experiments to assess direct and indirect effects of otter predation on aquatic communities.

 

Factors Affecting Migration and Recruitment in Headwater Fish Assemblages of Buffalo National River, AR

Funding Source: National Park Service
Project Duration: June 2001 to June 2003
Principal Investigator: DANIEL D. MAGOULICK
Graduate Research Assistant: SHAWN HODGES (M.S. Student)
MATT DEKAR (M.S. Student)
CHRIS BARE (M.S. Student)

Objectives:

1. Document the seasonal fluctuations in fish community species composition, relative abundance, and migration patterns at the macrohabitat, reach, and watershed scale.
2. Document larval drift densities and rates as components of fish community recruitment in the upstream, middle, and downstream reaches of Bear Creek.

Management Implications:

1. Information gained by this study will aid in interpreting the extent to which the Buffalo River depends upon Bear Creek and other tributaries to provide a fish species pool and as a potential spawning and nursery location.
2. Results will aid in interpreting the effects of barriers to fish movement, such as dams or stream drying.
3. Provide insight into the dynamics of fish reproduction and recruitment in intermittent and perennially interrupted streams.


Project Summary:

In July 1997, Searcy County Regional Water District (SCRWD) submitted a permit application to the Department of the Army Corps of Engineers for the construction of a dam in the headwaters of Bear Creek, a tributary to the Buffalo National River. In mid 1999, the Corps of Engineers denied the permit, but has recently overturned the ruling, suggesting that fish will be minimally impacted by a dam on Bear Creek. The Corps of Engineers' decision is based in part on the fact that Bear Creek becomes intermittent at some points during typical summers and the idea that fish in small, intermittent streams move little. However, the relationship between Buffalo River's fish community and the fish communities in the perennial and intermittent portions of Bear Creek are unknown. Likewise, the extent of dispersal and its impact on recruitment and fish assemblage structure within these stream systems is currently unknown.

 

The Influence of Stream flow and Habitat Variability on the
Fish Assemblage Structure of Arkansas Ozark Streams

Funding Source: National Park Service
Project Duration: June 2001 to June 2003
Principal Investigator: DANIEL D. MAGOULICK
Graduate Research Assistant: MANDY SCOTT (M.S. Student)

Objectives:

1. Determine the relationship between land clearing in the Arkansas Ozarks (on multiple spatial scales)and streamflow parameters in this region.
2. Examine differences in flood-resistance/resilience traits of fish between assemblages occurring in streams with differently variable flow and habitat complexity.
3. Determine the responses to high streamflow velocities of select species from these assemblages.
4. Develop and test a predictive model that describes fish assemblage structure in Arkansas Ozark streams according to each stream's flow regime and degree of habitat complexity.

Management implications:

1. Development of a predictive model that relates fish assemblage structure to land use and hydrology.
2. Ability to predict impacts of changes in land use on stream fish assemblages.

Project Summary:

Many biotic and abiotic factors have been found to influence fish assemblage structure in lotic systems. Abiotic factors are probably more influential in less stable systems because environmental variability keeps the fish populations from reaching a stable state where biotic interactions would be in control (Resh et al. 1988). Many Ozark Mountain streams fall into the unstable category because of their highly variable flow regimes, so it is likely that abiotic factors are shaping the fish assemblages in this region (Horwitz 1978). Streams can have highly variable flow regimes for many reasons, one of which is land use in the watershed. Clearing of land has led to higher peak flows (Verry et al. 1983), and increased overall discharge (Stednick 1996) in watersheds around the United States. Because much of the forested land in the Arkansas Ozarks is currently being cleared for agriculture, the impact on the hydrology and fish communities of these basins is an issue that should be addressed.

 

Interaction between the Introduced Crayfish, Orconectes neglectus, and native crayfish in the Spring River Drainage Basin

Funding Source: Causey Grant-in-Aid of Research- Department of Biological Sciences, University of Arkansas. Awarded to Michael Rabalais.
Project Duration: 1 July 2002 to 31 July 2004
Principal Investigator: DANIEL D. MAGOULICK
Graduate Research Assistant: MICHEAL R. RABALAIS (M.S. Student)

Research Objectives:

1. Test the hypothesis that environmental changes do not prevent O. eupunctus from surviving and growing in their former range.
2. Determine the extent of resource (habitat) partitioning between O. eupunctus and O. neglectus.
3. Test the hypothesis that introduced O. neglectus outcompete native O. eupunctus.

Management Implications:

1. Information from this study will help determine current impacts and predict future impacts of the invading crayfish species on native species.
2. Understanding mechanisms of displacement and effects of introduced crayfish on native species will allow managers to develop informed strategies regarding the need for mitigation and potential success of mitigation efforts.
3. Information gained here will be especially important in making decisions regarding the conservation of three species that are potentially at risk from this invasion, Orconectes eupunctus, (locally rare and uncommon and globally imperiled) Orconectes marchandi (both locally and globally imperiled), and Cambarus hubbsi.


Project Summary:

We recently found that the crayfish Orconectes neglectus has been introduced into the Spring River drainage of southern Missouri and northern Arkansas and appears to have the potential to negatively impact the native communities. We found that; 1) O. neglectus was introduced into portions of the South Fork Spring River between 1984 and 1998; 2) O. neglectus may be negatively impacting the native crayfish community, especially O. eupunctus, which is rare locally and globally and listed as special concern, as well as C. hubbsi; and 3) O. neglectus has the potential to spread in the Spring River drainage affecting other species including the imperiled O. marchandi. Field and laboratory research needs to be conducted to determine species displacement, mechanisms involved, and effects on ecosystem structure and function.